|
| Status |
Public on Apr 01, 2019 |
| Title |
Polarized B -cell functions |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we designed an in vitro approach to help us understand how extracellular components participate in human B cell functional plasticity and how this network might be involved in auto-immune diseases. Two activated T cell subsets were used to expose a common pool of B cells to distinct, controlled microenvironments. We showed that the same B cell population could experience two possible opposite evolutions, to either helper or suppressor functions, in a flexible manner. These cells were thus referred to as effector B cells (Eff B) or suppressor B cells (Supp B). We thus analyzed their different transcriptional programs by RNA sequencing.
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| |
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| Overall design |
B cells cultured with memory or naive T cells were analyzed by RNA sequencing
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| |
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| Contributor(s) |
Simon Q, Grasseau A, Hillion S |
| Citation(s) |
35359922 |
| |
| Submission date |
Mar 28, 2018 |
| Last update date |
Apr 07, 2022 |
| Contact name |
Sophie Hillion |
| E-mail(s) |
sophie.hillion@univ-brest.fr
|
| Phone |
33298223384
|
| Organization name |
INSERM U1227
|
| Lab |
Immunology
|
| Street address |
Felix le Dantec
|
| City |
Brest |
| ZIP/Postal code |
29609 |
| Country |
France |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (6)
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|
| Relations |
| BioProject |
PRJNA446049 |
| SRA |
SRP136642 |
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