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| Status |
Public on Mar 31, 2018 |
| Title |
Polycomb repressive complex 1 (PRC1) role in skin epithelium development [Epi Ring1a/b dKO] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Polycomb repressive complexes (PRC) 1 and 2 are essential chromatin regulators of cell identity. PRC1, a dominant executer of Polycomb-mediated control, functions as multiple sub-complexes that possess catalytic-dependent H2AK119 mono-ubiquitination (H2AK119ub) and catalytic-independent activities. Here, we show that despite its well-established repressor functions, PRC1 binds to both silent and active genes. Through in vivo loss-of-function studies, we show that global PRC1 function is essential for skin development and stem cell (SC) specification.
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| |
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| Overall design |
Newborn mouse epidermal cells were purified from Ring1a/b double knock out and control mice. RNA from the sorted cells was analyzed by RNA-seq.
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| |
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| Contributor(s) |
Cohen I, Zhao D, Zheng D, Ezhkova E |
| Citation(s) |
29727681 |
| |
| Submission date |
Mar 27, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Dejian Zhao |
| E-mail(s) |
dejian.zhao@yale.edu
|
| Organization name |
Yale School of Medicine
|
| Department |
Genetics
|
| Street address |
333 Cedar St
|
| City |
New Haven |
| State/province |
CT |
| ZIP/Postal code |
06510 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE112460 |
PRC1 fine-tunes gene repression and activation to safeguard skin development and stem cell specification |
|
| Relations |
| BioProject |
PRJNA445861 |
| SRA |
SRP136552 |
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