 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 26, 2018 |
| Title |
Knock-down of phosphoserine phosphatase gene effects rather N- than S-metabolism in Arabidopsis thaliana |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Elucidating the relative contribution of three serine biosynthesis pathways, i.e. (i) the glycolate pathway, (ii) the phosphorylated pathway and (iii) the glycerate pathway.
|
| |
|
| Overall design |
Arabidopsis thaliana plants [Wildtype Col-0 and three mutant lines in Col-0 background: (i) bou (At5g46800), (ii) psp17, (iii) psp18 (both: At1g18640)] were grown in biological triplicates under high CO2 (3000 ppm) for 35 days, 12 hr light / 12 hr dark and then shifted to ambient CO2 24 hours before harvesting. Samples for RNASeq were taken two hours before (“end of night”, EON) and two hours after (“beginning of day”, BOD) the onset of light.
|
| |
|
| Contributor(s) |
Samuilov S, Rademacher N, Brilhaus D, Altmann T, Eisenhut M, Rennenberg H, Weber AP |
| Citation(s) |
30619403 |
| |
| Submission date |
Mar 23, 2018 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Andreas PM Weber |
| E-mail(s) |
aweber@hhu.de
|
| Organization name |
Heinrich-Heine-University
|
| Department |
Institute for Plant Biochemistry
|
| Street address |
Universitätsstr. 1
|
| City |
Düsseldorf |
| ZIP/Postal code |
40225 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL21179 |
Illumina HiSeq 3000 (Arabidopsis thaliana) |
|
| Samples (18)
|
| GSM2301221 |
psp RNAi line 18, beginning of day_1 |
| GSM2301222 |
psp RNAi line 18, beginning of day_2 |
| GSM2301223 |
psp RNAi line 18, beginning of day_3 |
| GSM2301230 |
psp RNAi line 17, end of night_1 |
| GSM2301231 |
psp RNAi line 17, end of night_2 |
| GSM2301232 |
psp RNAi line 17, end of night_3 |
| GSM2301233 |
psp RNAi line 18, end of night_1 |
| GSM2301234 |
psp RNAi line 18, end of night_2 |
| GSM2301235 |
psp RNAi line 18, end of night_3 |
| GSM3063009 |
Wildtype, beginning of day_1 |
| GSM3063010 |
Wildtype, beginning of day_2 |
| GSM3063011 |
Wildtype, beginning of day_3 |
| GSM3063012 |
Wildtype, end of night_1 |
| GSM3063013 |
Wildtype, end of night_2 |
| GSM3063014 |
Wildtype, end of night_3 |
|
| Relations |
| BioProject |
PRJNA445392 |
| SRA |
SRP136326 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE112254_SupplementalDataset1.csv.gz |
10.9 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...