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Series GSE111824 Query DataSets for GSE111824
Status Public on May 07, 2019
Title Zfp281 shapes the transcriptome of trophoblast stem cells and is essential for placental development
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Placental development is a key event in mammalian reproduction and embryogenesis. However, the molecular basis underlying extraembryonic lineage specification and subsequent placental development is not fully understood. Through a genetic screen, we identified Zfp281 as a key factor for extraembryonic development. Disruption of Zfp281 in mice caused severe defects in extraembryonic as well as embryonic tissues. Importantly, Zfp281 was preferentially expressed in the trophoblast stem cell population in an FGF-dependent manner and ensured the expression of genes necessary for placental development. Through the analysis of transcriptome and epigenome, we identified Zfp281 as an important factor to shape the transcriptome of mammalian trophoblast stem cells.
Overall design To study the role of Zfp281 in transcriptional regulation, we performed RNA-seq using mouse and human TS cells. Furthermore, we performed H3K4me3 ChIP-seq and ATAC-seq to reveal the roles of Zfp281 in chromatin regulation.
Contributor(s) Ishiuchi T, Sato T, Ohishi H
Citation(s) 31067460
Submission date Mar 14, 2018
Last update date Jun 24, 2019
Contact name Takashi Ishiuchi
Organization name Kyushu University
Department Medical Institute of Bioregulation
Lab Epigenetics and Development
Street address 3-1-1 Maidashi, Higashi-ku
City Fukuoka
ZIP/Postal code 812-8582
Country Japan
Platforms (3)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (26)
GSM3040281 Zfp281 Het1_RNA-seq
GSM3040282 Zfp281 Het2_RNA-seq
GSM3040283 Zfp281 KO1_RNA-seq
BioProject PRJNA438284
SRA SRP135676

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SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE111824_RAW.tar 1.2 Gb (http)(custom) TAR (of BW, NARROWPEAK)
GSE111824_genes.fpkm_tracking.diff.txt.gz 1.0 Mb (ftp)(http) TXT
GSE111824_hTS_FPKM.txt.gz 371.6 Kb (ftp)(http) TXT
GSE111824_mTS_FPKM.txt.gz 331.6 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record
Processed data provided as supplementary file

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