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| Status |
Public on Oct 08, 2018 |
| Title |
TDP-43 modulates translation of specific mRNAs linked to neurodegenerative disease |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
We generated total mRNA libraries and ribosome footprint libraries from motor neuron-like cells (MN1) and primary cortical neurons expressing GFPor pure wild type cells as control, human TDP-43 wild type and mutant proteins to identify translational targets of human TDP-43 mutant protein
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| Overall design |
We used two replicates for MN1 cells and three replicates for primary neurons from which total mRNA and ribosome footprint libraries were generated. The libraries were multiplexed sequenced on an Illumina HiSeq2500 SR 50 base run.
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| Contributor(s) |
Neelagandan N, Gonnella G, Dang S, Alawi M, Indenbirken D, Grundhoff A, Kurtz S, Duncan KE |
| Citation(s) |
30357366 |
| |
| Submission date |
Mar 13, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Giorgio Gonnella |
| Organization name |
Universität Hamburg
|
| Department |
Zentrum für Bioinformatik
|
| Lab |
Genominformatik
|
| Street address |
Bundesstrasse 43
|
| City |
Hamburg |
| ZIP/Postal code |
20146 |
| Country |
Germany |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (28)
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| Relations |
| BioProject |
PRJNA438130 |
| SRA |
SRP135294 |
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