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| Status |
Public on Jun 14, 2018 |
| Title |
Opposing roles of miR-290 family and muscleblind-like proteins in the regulation of embryonic stem cell-specific alternative splicing and gene expression program |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
RNA profiling of WT,Dgcr8-/- mESCs and miR-294 or siMbnl1/2 transfected Dgcr8-/- ESCs showed that miRNAs, especially miR-294 regulate a large number of alternative splicing events, among which muscleblind-like proteins are responsible for a significant portion. Besides, upon depletion of miRNAs, RBPs and SFs are globally downregulated in mESCs.
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| Overall design |
Total RNA was analyzed from WT, Dgcr8-/- mESCs and Dgcr8 deficient mESCs transfected with miR-294 or siRNAs against Mbnl1/2.
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| |
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| Contributor(s) |
Wu D, Guo W, Gu K |
| Citation(s) |
29735517 |
| |
| Submission date |
Mar 12, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Yangming Wang |
| E-mail(s) |
yangming.wang@pku.edu.cn
|
| Phone |
0118610-62766945
|
| Organization name |
Peking University
|
| Department |
Institute of Molecular Medicine
|
| Lab |
Wang
|
| Street address |
5 Yiheyuan Road
|
| City |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA438001 |
| SRA |
SRP135104 |
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