|
|
GEO help: Mouse over screen elements for information. |
|
Status |
Public on Aug 27, 2018 |
Title |
The microARN-202 (miR-202) controls female fecundity by regulating oogenesis in medaka |
Organism |
Oryzias latipes |
Experiment type |
Expression profiling by array
|
Summary |
In fish, female fecundity is tightly linked to the proper completion of oogenesis that takes place in the ovary. These cellular processes have been shown to involve both endocrine and intra-ovarian factors. However, the role of small non-coding miRNAs remains largely unknown. Here, we analyzed the role of miR-202, a miRNA specifically expressed in the vertebrate gonads. We first used fluorescent in situ hybridization to determine the precise cellular expression of miR-202 in the medaka ovary. We then generated a mutant fish line (using CRISPR/Cas9 technology) to determine its role in female fecundity and oogenesis. We performed quantitative image analyses to analyze cellular modifications and a genome-wide transcriptomic approach to analyze gene expression modifications. Our results show that miR-202-5p is predominantly expressed in granulosa cells. In addition, mutant females display a drastically reduced fecundity. Our cellular and molecular analyses of ovaries from mutant females indicate that miR-202 deficiency impairs the early steps of oogenesis/folliculogenesis, which ultimately reduce female fecundity. This provides the first functional evidence that miR-202 is necessary for the female reproductive success, in particular the female fecundity, and shed new light on the regulatory mechanisms that control the early steps of follicular development.
|
|
|
Overall design |
Expression profiling of ovary from wild-type female and miR202 knock-out female
|
|
|
Contributor(s) |
Gay S, Bugeon J, Bouchareb A, Henry L, Montfort J, Le Cam A, Bobe J, Thermes V |
Citation(s) |
30199527 |
|
Submission date |
Mar 05, 2018 |
Last update date |
May 15, 2019 |
Contact name |
Aurelie LE CAM |
E-mail(s) |
aurelie.le-cam@inrae.fr
|
Organization name |
INRA
|
Department |
PHASE
|
Lab |
LPGP
|
Street address |
Campus de Beaulieu
|
City |
Rennes |
ZIP/Postal code |
35042 |
Country |
France |
|
|
Platforms (1) |
GPL24100 |
Agilent-082421 Oryzias latipes Medaka_Genome83_Mitochondrie |
|
Samples (8)
|
|
Relations |
BioProject |
PRJNA436905 |
Supplementary file |
Size |
Download |
File type/resource |
GSE111388_RAW.tar |
75.2 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
|
|
|
|
|