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Series GSE11132 Query DataSets for GSE11132
Status Public on Jun 10, 2008
Title 22RV1 prostate cancer cell line transfected with SPINK1 siRNA vs control siRNA
Organism Homo sapiens
Experiment type Expression profiling by array
Summary ETS gene fusions have been characterized in a majority of prostate cancers, however key molecular alterations in ETS negative cancers are unclear. Here we used an outlier meta-analysis (meta-COPA) to identify SPINK1 outlier-expression exclusively in a subset of ETS rearrangement negative cancers (~10% of total cases). We validated the mutual exclusivity of SPINK1 expression and ETS fusion status, demonstrated that SPINK1 outlier-expression can be detected non-invasively in urine and observed that SPINK1 outlier-expression is an independent predictor of biochemical recurrence after resection. We identified the aggressive 22RV1 cell line as a SPINK1 outlier-expression model, and demonstrate that SPINK1 knockdown in 22RV1 attenuates invasion, suggesting a functional role in ETS rearrangement negative prostate cancers.
Keywords: Genetic Modification
Overall design 22RV1 cells were infected with non-targeting siRNA or siRNA against SPINK1. For reported hybridizations, the reference channel is 22RV1 cells infected with non-targeting siRNA. Duplicate hybridizations were performed with duplicate dye flips, for a total of four arrays. Over and under-expressed signatures were generated by filtering to include only features with significant differential expression (PValueLogRatio < 0.01) in all hybridizations and Cy5/Cy3 ratios > or < 1 in all hybridizations.
Contributor(s) Tomlins SA, Chinnaiyan AM
Citation(s) 18538735
Submission date Apr 10, 2008
Last update date Feb 22, 2018
Contact name Scott Tomlins
Phone 734-615-1417
Organization name University of Michigan
Department Pathology
Lab Chinnaiyan Lab
Street address 1400 E. Medical Center Dr., 5410 CCGC
City Ann Arbor
State/province MI
ZIP/Postal code 48109
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (4)
GSM280793 22RV1 spink1 sirna rep1
GSM280794 22RV1 spink1 sirna rep2
GSM280795 22RV1 spink1 sirna dye rep1
BioProject PRJNA106919

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Supplementary file Size Download File type/resource
GSE11132_RAW.tar 62.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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