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| Status |
Public on Apr 16, 2019 |
| Title |
Next Generation Sequencing analysis of Hes1+/+ Cre-ERT2 and Hes1fl/fl Cre-ERT2 bone marrow derived macrophages transcriptomes. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To evaluate the potential role of Hes1 in regulation of type I IFNs and the downstream ISGs expression, we generated the bone marrow-derived macrophages (BMDMs) from Hes1+/+ Cre-ERT2 and Hes1fl/fl Cre-ERT2 mice. RNA-sequencing analysis of Poly(I:C) [(polyinosinic: polycytidylic acid), a mimic of viral double-stranded RNA]-treated BMDMs revealed that 49 genes were induced more than two-fold by Poly(I:C) for 3 hrs in Hes1-deficient BMDMs relative to their expression in Hes1+/+Cre-ERT2 cells. Notably, 67.3% of these upregulated genes were ISGs by screening the Interferome database, suggesting that Hes1 inhibits expression of TLR-mediated ISGs,these results indicated that Hes1 negatively regulates TLR3-mediated induction of ISGs in macrophages.
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| |
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| Overall design |
mRNA profiles of BMDMs from Hes1+/+ Cre-ERT2 and Hes1fl/fl Cre-ERT2 mice with or without Poly(I:C) treatment for 1 or 3 hours.
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| Contributor(s) |
Ning F, Zhang B |
| Citation(s) |
31015298 |
| |
| Submission date |
Feb 27, 2018 |
| Last update date |
Jul 17, 2019 |
| Contact name |
Fei Ning |
| E-mail(s) |
ningff@yeah.net
|
| Organization name |
Institute for Immunology
|
| Street address |
Tsinghua Yuan Street
|
| City |
Beijing |
| ZIP/Postal code |
100084 |
| Country |
China |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA436091 |
| SRA |
SRP133557 |
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