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| Status |
Public on Jun 20, 2018 |
| Title |
mRNA profiles of active hematopoieitc stem cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study are to elucidate the underlying mechanism for the activation of HSCs
Methods: After wild type (WT) HSCs (CD150+ CD48- EPCR+ Lineage-) were treated with 5-fluorouracil (5-FU), 100 cells of HSCs were sorted. Moreover, after the culture with or without Nifedipien, cultured HSC fraction (CD150+ CD48- EPCR+ c-kit+ Sca-1+ Lineage-) were sorted. These cells were subjected to mRNA sequence using Next-seq (n>3). The sequence reads that passed quality filters were analyzed by CLC genomic workbench.
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| Overall design |
After the culture with or without Nifedipine, HSCs wihtin 3 cell division population were sorted, and subsequently mRNA profiles were generated by deep sequencing using Next-seq (n>3).
Using HSCs derived from untreated and 5-FU-treated mice, mRNA profiles were generated by deep sequencing using Next-seq (n>4).
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| Contributor(s) |
Umemoto T, Suda T |
| Citation(s) |
29946000 |
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| Submission date |
Feb 26, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Terumasa Umemoto |
| Organization name |
Kumamoto University
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| Street address |
2-2-1, Honjo, Chuo-Ku
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| City |
Kumamoto |
| ZIP/Postal code |
862-0957 |
| Country |
Japan |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (15)
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| Relations |
| BioProject |
PRJNA435963 |
| SRA |
SRP133481 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE111118_RAW.tar |
11.3 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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