|
| Status |
Public on Mar 01, 2018 |
| Title |
Glucagon contributes to liver zonation in mice [Gcg KO] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Liver zonation characterizes the separation of metabolic pathways along the lobules and is required for optimal function. Wnt/beta-catenin signaling controls metabolic zonation by activating genes in the perivenous hepatocytes, while suppressing genes in the periportal counterparts. We now demonstrate that glucagon opposes the actions of Wnt/beta-catenin signaling on gene expression and metabolic zonation pattern. The effects were more pronounced in the periportal hepatocytes where 28% of all genes were activated by glucagon and inhibited by Wnt/beta-catenin. The glucagon and Wnt/beta-catenin receptors and their signaling pathways are uniformly distributed in periportal and perivenous hepatocytes and the expression is not regulated by the opposing signal. Collectively, our results show that glucagon controls gene expression and metabolic zonation in the liver through a counter play with the Wnt/beta-catenin signaling pathway.
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| |
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| Overall design |
Gene expression profiling of Gcg-/- and wild type mice in liver
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| |
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| Contributor(s) |
Cheng X, Xin Y, Gromada J |
| Citation(s) |
29555772 |
| |
| Submission date |
Feb 15, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Yurong Xin |
| E-mail(s) |
yurong.xin@regeneron.com
|
| Organization name |
Regeneron Pharmaceuticals, Inc.
|
| Street address |
777 Old Saw Mill River Road
|
| City |
Tarrytown |
| State/province |
NY |
| ZIP/Postal code |
10598 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE110674 |
Glucagon contributes to liver zonation in mice |
|
| Relations |
| BioProject |
PRJNA434234 |
| SRA |
SRP132927 |
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