|
| Status |
Public on Feb 12, 2019 |
| Title |
Identification of transcriptional network regulated by FGFR1 fusion kinase in stem cell leukemia and lymphoma (SCLL) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Oncogenic transformation of hematopoietic stem cells by chimeric fusion kinases causing constitutive activation of FGFR1 leads to a stem cell leukemia/lymphoma (SCLL) syndrome, accompanied by widespread dysregulation of gene activity. The goals of this study are to use NGS-derived transcriptome profiling (RNA-seq) to identify genes regulated by FGFR1 fusion kinase in SCLL.
Methods: Three different cell models for SCLL, respectively BBC2 (B cell lymphoblastic leukemia/lymphoma), ZNF112 (B cell lymphoblastic leukemia/lymphoma) and BCRF8C (myloid leukemia) were treated with selective FGFR1 kinase inhibitor,then the total RNA from treatment and viechel control were isolated for RNA-Seq analysis.
Results: A global gene experssion change is generated, and provides fundamental information for demostration of molecular mechanisms in FGFR1 driven leukemogenesis.
|
| |
|
| Overall design |
comparasion of the transcriptomes of SCLL lines with and without the inhibition of FGFR1
|
| |
|
| Contributor(s) |
Cowell J, Hu T |
| Citation(s) |
29720732, 31628193 |
| |
| Submission date |
Feb 12, 2018 |
| Last update date |
Oct 23, 2019 |
| Contact name |
John Cowell |
| E-mail(s) |
jcowell@augusta.edu
|
| Phone |
7067214381
|
| Organization name |
Augusta University
|
| Street address |
1410 Laney-Walker Blvd
|
| City |
Augusta |
| State/province |
GA |
| ZIP/Postal code |
30912 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA433808 |
| SRA |
SRP132677 |
Less...
More...