|
| Status |
Public on Feb 09, 2019 |
| Title |
Exploring the effects of different sample preparation factors on RNA sequencing |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated the effects of different sample preparation factors on RNA-seq experiments, including RNA concentration, library storage time, and cryopreserved condition, by comparing their sequencing biases, gene expression profiles, and biological function using primary B cell and CD4+ cell blood samples in healthy subjects.
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| |
|
| Overall design |
The effects of RNA concentration, library storage time, and cryopreserved condition for RNA-seq experiments
|
| |
|
| Contributor(s) |
Pease LR, Zhang Y |
| Citation(s) |
30529531 |
| |
| Submission date |
Feb 09, 2018 |
| Last update date |
May 21, 2019 |
| Contact name |
Yuji Zhang |
| E-mail(s) |
yuzhang@som.umaryland.edu
|
| Phone |
410-706-8523
|
| Organization name |
University of Maryland
|
| Street address |
660 W. Redwood Street
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
|
| Samples (29)
|
|
| Relations |
| BioProject |
PRJNA433678 |
| SRA |
SRP132594 |
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