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| Status |
Public on Feb 09, 2018 |
| Title |
The protective effect of celastrol in ANIT-induced cholestasis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: The goals of this study was to (1) evaluate the protective effect of celastrol on alpha-naphthylisothiocyanate (ANIT)-induced cholestasis and (2) which genes were recovered by celastrol.
Methods:To investigate the protective effect of celastrol on ANIT-induced cholestasis, the WT mice were randomly assigned into two groups, respectively (n=3): (1) ANIT; (2) ANIT+Celastrol. ANIT+Celastrol group was orally treated with celastrol (10 mg/kg dissolved in 1% DMSO + 2% Tween 80 + 97% water) for 5 consecutive days. After celastrol was treated for 3 days, ANIT and ANIT+Celastrol groups were given a single oral dose of ANIT. All mice were killed 48 h after ANIT administration. Liver samples were harvested and frozen at -80 °C before analysis.
Results: A total of 978 DEGs were identified. Large numbers of these DEGs were related to activation of SIRT1, which included increased FXR signaling and inhibition of PPARγ, nuclear factor-kappa B (NF-κB), and P53 signaling.
Conclusions: Celastrol could protect ANIT-induced cholestasis by recovering disrupted Sirt1 level.
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| Overall design |
6 hepatic mRNA profiles were generated by deep sequencing, in triplicate, using Illumina GAIIx.
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| Contributor(s) |
Zhao Q, Li F |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Feb 08, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Qi Zhao |
| E-mail(s) |
zhaoqi1@mail.kib.ac.cn
|
| Phone |
13529147951
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| Organization name |
Kunming Institute of Botany, Chinese Academy of Sciences
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| Street address |
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China
|
| City |
云南 |
| ZIP/Postal code |
650201 |
| Country |
China |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA433590 |
| SRA |
SRP132536 |
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