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Series GSE110297 Query DataSets for GSE110297
Status Public on Jul 19, 2018
Title Cardiolipin Synthesis Governs Systemic Energy Homeostasis through Thermogenic Fat Mitochondria
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Here we show that synthesis of the mitochondrial phospholipid cardiolipin is an indispensable driver of thermogenic fat function. Cardiolipin biosynthesis is robustly induced in brown and beige adipose upon cold exposure. Mimicking this response by overexpressing cardiolipin synthase (Crls1) enhances energy consumption in mouse and human adipocytes. Crls1 deficiency diminishes mitochondrial uncoupling in brown and beige adipocytes and elicits a nuclear transcriptional response through ER stress-mediated retrograde communication. Cardiolipin depletion in brown and beige fat abolishes adipose thermogenesis and glucose uptake and renders animals strikingly insulin resistant. We further identify a rare human CRLS1 variant associated with insulin resistance and show that adipose CRLS1 levels positively correlate with insulin sensitivity. Thus, adipose cardiolipin is a powerful regulator of organismal energy homeostasis through thermogenic fat bioenergetics.
Overall design Genome-wide profiling of mRNA from brown adipose tissue (BAT) of five different mice of either wild-type (WT) or with knockout of CRLS1 (KO)
Contributor(s) Sustarsic EG, Forss I, Mandrup S, Gerhart-Hines Z
Citation(s) 29861389
Submission date Feb 07, 2018
Last update date Sep 12, 2018
Contact name Susanne Mandrup
Phone +45 6550 2340
Organization name University of Southern Denmark
Department Biochemistry and Molecular Biology
Street address Campusvej 55
City Odense M
ZIP/Postal code 5230
Country Denmark
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (10)
GSM2985497 WT rep 1
GSM2985498 WT rep 2
GSM2985499 WT rep 3
BioProject PRJNA433399
SRA SRP132399

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Supplementary file Size Download File type/resource
GSE110297_All_genes_NormalizedReadsPerKb.txt.gz 2.1 Mb (ftp)(http) TXT
GSE110297_Genes_down_with_KO_NormalizedReadsPerKb.txt.gz 477.1 Kb (ftp)(http) TXT
GSE110297_Genes_up_with_KO_NormalizedReadsPerKb.txt.gz 444.6 Kb (ftp)(http) TXT
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