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| Status |
Public on Mar 19, 2019 |
| Title |
Obesity expands a distinct population of adipose-resident T cells and increases vulnerability to infection |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Obesity in humans is associated with worsened health outcomes following infections compared to non-obese individuals. Here, we examined the effects of adipose tissue and obesity on T cell responses to virus infection in mice. Lymphocytic choriomeningitis virus (LCMV) grew to high titer in adipose tissue. Virus-specific T cells enter the adipose tissue to resolve infection but then remain as a memory population distinct from memory T cells in lymphoid tissues. Memory CD4+ and CD8+ T cells residing in adipose were abundant, accounting for roughly 10% of all virus-specific memory T cells. Diet-induced obesity increased memory T cell number in adipose and spleen. Upon re-challenge infection, memory T cells caused severe pathogenesis, leading to sharply increased lipase levels, calcification of adipose tissue, pancreatitis, and reduced survival in obese mice but not lean mice. Thus, obesity leads to a unique form of viral pathogenesis involving memory T cell-dependent adipocyte destruction and collateral damage to other tissues.
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| Overall design |
RNA-seq comparison of CD4+ T cells in spleen vs fat (with 10 mice pooled per replicate) and CD8+ T cells in spleen vs fat (with 5 mice pooled per replicate)
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| Contributor(s) |
Ichiro M, Joshua S, Melinda B, Terry M, Jason W |
| Citation(s) |
30970254 |
| |
| Submission date |
Feb 06, 2018 |
| Last update date |
Jun 25, 2019 |
| Contact name |
Joshua Starmer |
| Organization name |
University of North Carolina at Chapel Hill
|
| Department |
Genetics
|
| Lab |
Magnuson
|
| Street address |
120 Mason Farm Road
|
| City |
Chapel Hill |
| State/province |
NORTH CAROLINA |
| ZIP/Postal code |
27599-7264 |
| Country |
USA |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA433181 |
| SRA |
SRP132290 |
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