|
| Status |
Public on Dec 26, 2018 |
| Title |
T cells activated in the presence or absence of TLR agonists |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the gene expression differences between OT-1 T cells activated alone or in the presence of TLR1/2 (Pam3CSK4) or TLR7 (Gardiquimod). By activating OT-1 splenocytes in the presence or absence of the TLR agonists, isolating RNA from purified CD8+ T cells we show that cells activated in the presence of either TLR1/2 or TLR7 agonists had similar transcriptional profiles demonstrating an increase in Th1 cytokine expression over cells that were activated alone. This study provides a key piece of information for those designing T cell mediated therapies.
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| |
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| Overall design |
Examintation of T cells activated in the presnece or absence of TLR agonists
|
| |
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| Contributor(s) |
McIlwain SJ, McNeel DG, Zahm CD, Ong IM |
| Citation(s) |
30201735 |
| |
| Submission date |
Feb 05, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Douglas McNeel |
| E-mail(s) |
dm3@medicine.wisc.edu
|
| Phone |
(608) 263-4198
|
| Organization name |
University of Wisconsin
|
| Street address |
111 Highland Ave., Rm 7007
|
| City |
Madison |
| State/province |
Wisconsin |
| ZIP/Postal code |
53704 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (24)
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|
| Relations |
| BioProject |
PRJNA432950 |
| SRA |
SRP132195 |
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