|
| Status |
Public on Jan 08, 2019 |
| Title |
Impaired TNFa-driven dendritic cell activation limits LPS-induced protection from asthma in infants. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We found that approximately 200 genes were differentially expressed between CD11b+ lung-migratory dendritic cells (CD11b+ mDCs) from adult and infant mice treated with house dust mite allergen + LPS, and that the TNFa-via NFkB signaling pathway was significantly enriched in CD11b+ mDCs from adults relative to those from infants. Specifically, out of the 200 genes in the hallmark TNFa-via NFkB signaling pathway that were analyzed, 31 genes were significantly downregulated in CD11b+ mDCs from infants compared to CD11b+ mDCs from adults.
|
| |
|
| Overall design |
We conducted an RNA-seq analysis on sorted CD11b+ lung-migratory dendritic cells (CD11b+ mDCs: B220-CD64-CD103-CD11c+MHCIIhiCD11b+) from adult (7wks-old) and infant (2-3wks-old) mice obtained from the mediastinal lymph node (mLN) 24 hours after house dust mite allergen (HDM) +LPS intranasal sensitization. This analysis was performed to identify and quantify differentially expressed genes and differentially enriched signaling pathways between CD11b+ mDCs from adults and infants.
|
| |
|
| Contributor(s) |
Kaur K, Bachus H, Marquez-Lago TT, Papillion AM, Ballesteros-Tato A, León B |
| Citation(s) |
30635238 |
| |
| Submission date |
Feb 02, 2018 |
| Last update date |
Apr 09, 2019 |
| Contact name |
Beatriz Leon Ruiz |
| E-mail(s) |
bleon@uab.edu
|
| Phone |
(205) 975-4806
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Microbiology
|
| Lab |
Shelby 406
|
| Street address |
1825 University Blvd
|
| City |
Birmingham |
| State/province |
Alabama |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (7)
|
|
| Relations |
| BioProject |
PRJNA432657 |
| SRA |
SRP132040 |
Less...
More...