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| Status |
Public on Jul 10, 2019 |
| Title |
Real-time quantitative PCR analysis of human cell-free circulating miRNAs expression in early rheumatoid arthritis |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
The second metacarpal head (MCP2) of the 20 ERA patients and 6 sex- and age- matched healthy controls were scanned by HR-pQCT. Then the 20 ERA patients were devided into two sub-groups: bone erosion group and non-erosion group detected by HR-pQCT.
Peripheral blood samples were collected using Ethylenediaminetetraacetic acid (EDTA) at the day of fasting status of ERA patients as well as HCs and were centrifuged at 3000×g for 10 minutes at room temperature. Then, the plasma samples were aliquoted and centrifuged at 3000×g for an additional 10 minutes at 4oC to remove any remaining cellular debris. The plasma was immediately stored at –80oC until analysis.
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| Overall design |
miRNA were extracted from peripheral blood samples using EDTA in ten treatment-naïve ERA patients with maximal erosion volume at MCP2 detected by HR-pQCT, another ten treatment-naïve ERA patients without erosion and six age- and sex-matched healthy controls (HCs) as indicated in the summary. miRNA profiling was performed by TaqMan RT-qPCR (Life Technologies) according to the manufacturer’s instructions. This method allows the simultaneous analysis of 377 unique miRNAs (Human Pools A V2.1) using 384-well Cards.
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| Contributor(s) |
Tam L, Yue J |
| Citation(s) |
31273939 |
| |
| Submission date |
Jan 30, 2018 |
| Last update date |
Jul 10, 2019 |
| Contact name |
Lai-Shan TAM |
| Organization name |
The Chinese University of Hongkong
|
| Department |
Department of Medicine and Therapeutics, Prince of wales hospital
|
| Lab |
Division of Rheumatology
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| Street address |
30-32 Silver City Street, Shatin
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| City |
Hong Kong |
| State/province |
Hong Kong |
| ZIP/Postal code |
000000 |
| Country |
Hong Kong |
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| Platforms (1) |
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| Samples (26)
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| Relations |
| BioProject |
PRJNA432241 |
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