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Series GSE109808 Query DataSets for GSE109808
Status Public on Jan 30, 2018
Title Genome-wide CRISPR screen identifies TSC1 and DEPDC5 that control HIV-1 latency via the mTOR Signaling pathway
Organism Homo sapiens
Experiment type Other
Summary Identification of factors that control HIV-1latency
Overall design CRISPR activation technology was used to identify genes that control HIV-1 latency. The complete sgRNA library (plasmid sample) were packaged into lentivirus and used to transduce C11 cells (transduced sample). The proportion of GFP positive C11 cells increased after infection with CRISPRV2.0. GFP positive cells were sorted for 4 rounds and enriched genes. In three independent biological replicates( samples Screen1, 2, and 3), the sgRNAs enriched in Initial,GFP negative andGFP postive populations were sequenced.
Contributor(s) Jin S, Zhang X
Citation(s) 30087333
Submission date Jan 29, 2018
Last update date Dec 23, 2018
Contact name SHAN JIN
Phone 13122601610
Organization name FUDAN University
Street address Scientific Research Center 2901 Cao Lang Road, Jin Shan District, Shanghai, P.R.China
City Shanghai
ZIP/Postal code 201508
Country China
Platforms (1)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (9)
GSM2970249 Screen1-4round GFP Initial
GSM2970250 Screen1-4round GFP negative
GSM2970251 Screen1-4round GFP positive
BioProject PRJNA432068
SRA SRP131690

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE109808_RAW.tar 2.9 Mb (http)(custom) TAR (of TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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