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| Status |
Public on Jan 26, 2018 |
| Title |
Differential gene expressions of human pluripotent stem cells derived cells in different culture system |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Human pluripotent stem cells (H9s) were differentiated into endothelial cells (ECs), neural stem cells (NSCs) and vascular smooth muscle cells (VSMCs) in 2 dimentional, 3 dimensional PEG hydrogel and alginate hydrogel culture system, and we compared their differential gene expression in different culture system, respectively.
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| |
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| Overall design |
RNA deep sequencing
H9s were maintained in E8 medium for routine culture. Then H9s were differentiated into ECs, NSCs and VSMCs in 2D, 3D PEG hydrogel and alginate hydrogel cell culture system according to their differentiation protocol. mRNAs were sequenced.
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| |
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| Contributor(s) |
Yuguo L, Haishuang L, Chi Z |
| Citation(s) |
30527760 |
| |
| Submission date |
Jan 25, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Chi Zhang |
| E-mail(s) |
czhang5@unl.edu
|
| Organization name |
University of Nebraska - Lincoln
|
| Street address |
1901 Vine Street
|
| City |
Lincoln |
| State/province |
NE |
| ZIP/Postal code |
68588 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA431651 |
| SRA |
SRP131419 |
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