|
| Status |
Public on Oct 25, 2018 |
| Title |
Hemogenic Endothelium transcriptome along the timeline of hESC differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The differentiation of human embryonic stem cells to hematopoietic lineages initiates with the specification of hemogenic endothelium, a transient specialized endothelial precursor of all blood cells.Unfortunately, absence of hemogenic endothelium-specific markers as well as lack of consensus in the timing of hemogenic potential analysis and methodologies used to study the hematopoietic potential of this precursor prevents reaching clear and definite conclusions. Here, we demonstrate that the hemogenic potential of the endothelium precursor population sharply decline over the course of the differentiation process.
Poly(A) RNA-sequencing on CD31+CD144+ population at day 6, day 8 and day 10 of EB diffferentiation with or without the addition of cytokines. Comparasion with hematopoietic committed population CD31+CD144- from day 10 of EB differentiation.
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| |
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| Overall design |
CD31+CD144+ signature along the EB differentiation
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| |
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| Contributor(s) |
Garcia-Alegria E, Menegatti S, Fadlullah MZ, Menendez P, Kouskoff V, Lacaud G |
| Citation(s) |
30449319 |
| |
| Submission date |
Jan 25, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Muhammmad Zaki Fadlullah Wilmot |
| E-mail(s) |
muhammad.fadlullahwilmot@manchester.ac.uk
|
| Organization name |
Cancer Research UK Manchester Institute
|
| Street address |
Wilmslow Road
|
| City |
manchester |
| ZIP/Postal code |
M204BX |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (14)
|
| GSM2947852 |
CD31posCD144pos day 8 no CK replicate 2 |
| GSM2947853 |
CD31posCD144pos day 8 with CK replicate 1 |
| GSM2947854 |
CD31posCD144pos day 8 with CK replicate 2 |
| GSM2947855 |
CD31posCD144pos day 10 no CK replicate 1 |
| GSM2947856 |
CD31posCD144pos day 10 no CK replicate 2 |
| GSM2947857 |
CD31posCD144pos day 10 with CK replicate 1 |
| GSM2947858 |
CD31posCD144pos day 10 with CK replicate 2 |
| GSM2947859 |
CD31posCD144neg day 10 no CK replicate 1 |
| GSM2947860 |
CD31posCD144neg day 10 no CK replicate 2 |
| GSM2947861 |
CD31posCD144neg day 10 with CK replicate 1 |
| GSM2947862 |
CD31posCD144neg day 10 with CK replicate 2 |
|
| Relations |
| BioProject |
PRJNA431592 |
| SRA |
SRP131202 |
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