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| Status |
Public on Mar 12, 2018 |
| Title |
Epigenetic Rescue of Autism-like Social Deficits in Shank3-Deficient Mouse Models |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine whether histone deacetylase (HDAC) inhibition has genome-wide effects on gene expression, we used RNA sequencing to analyze the mRNA profile in prefrontal cortex (PFC) of wild-type (WT) and Shank3-deficient (Shank3+/ΔC) mice treated with romidespin or saline control. We mapped the sequences to 24,420 mouse genes. Compared to WT, 365 genes in saline-treated Shank3+/ΔC mice showed a change in expression values of at least 1.2 fold and p ≤ 0.01 (213 downregulated, 152 upregulated). In romidepsin-treated Shank3+/ΔC mice, ~88% of the downregulated genes (n=187 genes) were normalized to control values. Further analysis of the romidepsin-restored genes revealed enrichment in actin cytoskeleton-mediated transport, signal transduction pathways, and developmental processes.
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| Overall design |
Comparison of gene expression in prefrontal cortex of wild-type mice, saline-treated Shank3-deficient mice and romidepsin-treated mice
Heterozygous Shank3+/ΔC mice (5-8 weeks old, male) expressing C-terminal (exon 21) deleted Shank3 (Jackson Labs), which exhibited the significant loss of full-length Shank3 expression, and age-matched wild-type littermates (male), were used in the experiments.
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| Contributor(s) |
Qin L, Ma K, Wang Z, Hu Z, Yan Z |
| Citation(s) |
29531362 |
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| Submission date |
Jan 17, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Zhen Yan |
| E-mail(s) |
zhenyan@buffalo.edu
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| Phone |
(716) 829-3058
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| Organization name |
University at Buffalo
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| Department |
Physiology and Biophysics
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| Street address |
124 Sherman Hall
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| City |
Buffalo |
| State/province |
NY |
| ZIP/Postal code |
14214 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA430439 |
| SRA |
SRP131096 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109328_RAW.tar |
800.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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