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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jan 18, 2018 |
| Title |
Small molecule screen identifies de novo nucleotide synthesis as a vulnerability of cells lacking SIRT3 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Sirtuin 3 (SIRT3) is an NAD+-dependent deacetylase downregulated in aging and age-associated diseases such as cancer and neurodegeneration, and high fat diet (HFD)-induced metabolic disorders. Thus, we performed a small molecule screen and identified an unexpected metabolic vulnerability associated with SIRT3 loss.
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| Overall design |
RNA sequencing in SV40T immortalized SIRT3 WT (triplicates) and SIRT3 KO MEF (duplicates) lines under normal conditions.
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| Contributor(s) |
Gonzalez Herrera KN, Zaganjor E, Ishikawa Y, Spinelli JB, Yoon H, Lin J, Satterstrom FK, Ringel A, Mulei S, Souza A, Gorham JM, Benson CC, Seidman JG, Sorger PK, Clish CB, Haigis MC |
| Citation(s) |
29466723 |
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| Submission date |
Jan 17, 2018 |
| Last update date |
Jun 26, 2019 |
| Contact name |
Karina Noemi Gonzalez Herrera |
| E-mail(s) |
knherrer@fas.harvard.edu
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| Organization name |
Harvard Medical School
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| Department |
Cell Biology
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| Lab |
Haigis Lab
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| Street address |
240 Longwood Avenue, LHRRB, Haigis Lab, Room 301
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA430334 |
| SRA |
SRP131032 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE109279_DESeq2.DiffExp_WT_SIRT3KO_MEFs.txt.gz |
1.5 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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