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| Status |
Public on Jun 09, 2018 |
| Title |
Effect of BRD4 deletion on the Transcriptome of Different Thymocyte Subpopulations |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
By conditionally deleting BRD4 at various stages of thymic differentiation, we have established that BRD4 deficiency selectively affects a unique developmental subpopulation of thymocytes.
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| Overall design |
We examined by RNA-seq the effect on gene expression of BRD4 deletion in ex vivo DN, ISP, DP, CD4 and CD8 thymocyte subpopulations.
The analysis was also performed on WT or BRD4 deleted ISP and DP thymocytes cultured for 16 hours at 37oC
In this analysis, the conditional deletion of BRD4 (cKO) is achieved using the LCK-cre Transgene.
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| Contributor(s) |
Gegonne A, Chen Q, Meerzaman D, Singer D |
| Citation(s) |
29972774 |
| |
| Submission date |
Jan 16, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Dinah S. Singer |
| E-mail(s) |
dinah.singer@nih.gov
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| Phone |
301-496-9097
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| Organization name |
National Cancer Institute
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| Department |
Division of Cancer Biology
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| Street address |
Building 10, Room 4B-17
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (38)
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| Relations |
| BioProject |
PRJNA430246 |
| SRA |
SRP131016 |
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