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| Status |
Public on Nov 06, 2018 |
| Title |
C1 single-cell RNA-seq of Adult ID4-EGFP mouse spermatoognia |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To reveal distinct transcriptomes associated with spermatogonial stem cell renewal vs. initiation of differentiation, single-cell transcriptomes from Adult ID4-EGFP+ spermatogonia were subdivided into subpopulations that displayed distinct fates when assayed by transplantation, with ID4-EGFPbright cells highly enriched for SSCs, and ID4-EGFPdim cells enriched for progenitors. We used the Fluidigm C1 instrument to capture individual spermatogonia for SMART-Seq2 single-cell RNA-seq.
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| Overall design |
Four replicate preparations of Adult mouse ID4-EGFP+ spermatogonia were used for this study. Data are from a total of 300 cells. Cells were binned into quartiles according to EGFP epifluorescence intensity (Q1 = EGFP-bright, Q4=EGFP-dim, Q2 and Q3 have intermediate EGFP fluorescence) to facilitate comparisons.
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| Contributor(s) |
Hermann BP, Cheng K, Singh A, Roa-de-la-Cruz L, Mutoji KN, Gildersleeve H, Chen I, Mayo M, Westernströer B, Law NC, Oatley MJ, Velte EK, Niedenberger BA, Fritze D, Silber S, Geyer CB, Oatley JM, McCarrey JR |
| Citation(s) |
30404016 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R00 HD062687 |
Transcriptional regulatory networks in spermatogonial stem cells |
THE UNIVERSITY OF TEXAS AT SAN ANTONIO |
Brian Peter Hermann |
| R01 HD090007 |
Origin and functional significance of the spermatogonial stem cell transcriptome barcode |
THE UNIVERSITY OF TEXAS AT SAN ANTONIO |
Brian Peter Hermann |
| P30 GM092334 |
Recruitment of New Stem Cell Biologist |
THE UNIVERSITY OF TEXAS AT SAN ANTONIO |
John R MCCARREY |
| R01 HD078679 |
Epimutations in Offspring Produced by Assisted Reproductive Technologies (ART) |
THE UNIVERSITY OF TEXAS AT SAN ANTONIO |
John R MCCARREY |
| R01 HD090083 |
The role of retinoid exposure in specification of the foundational SSC pool |
EAST CAROLINA UNIVERSITY |
Chris Bennett Geyer |
| R01 HD061665 |
Control of Spermatogonial Stem Cell Fate Decisions by HLH Factors |
Washington State University |
Jon M Oatley |
| G12 MD007591 |
RCMI Center for Interdiscplinary Health Research CIHR |
THE UNIVERSITY OF TEXAS AT SAN ANTONIO |
George Perry |
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| Submission date |
Jan 09, 2018 |
| Last update date |
Mar 21, 2019 |
| Contact name |
Brian P. Hermann |
| E-mail(s) |
Brian.Hermann@utsa.edu
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| Phone |
210-458-8047
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| Organization name |
University of Texas at San Antonio
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| Department |
Department of Biology
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| Street address |
1 UTSA Circle
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| City |
San Antonio |
| State/province |
TX |
| ZIP/Postal code |
78249 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (300)
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| Relations |
| BioProject |
PRJNA429266 |
| SRA |
SRP128582 |
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