|
| Status |
Public on Aug 31, 2018 |
| Title |
Nuclear miRNA-mediated gene silencing dependent on Argonaute proteins is required for embryonic stem cell differentiation [1_Differentiation_rna_seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here, we show that embryonic stem cells (ESCs) and muscle stem cells contain high levels of nuclear AGO proteins and that in ESCs nuclear AGO2 protein activity is sufficient to allow for differentiation. Transcriptome-wide mapping of binding sites at nucleotide resolution revealed that nuclear AGO2 expands its target sequence repertoire in a miRNA-dependent manner to include intronic sequences to induce post-transcriptional gene silencing
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| Overall design |
LIF withdrawal and ESC differentiation for 6 days with WT ESC and ESC expressing NLS-AGO2
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| |
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| Contributor(s) |
Sarshad AA, Juan AH, Correa Muler AI, Anastasakis D, Wang X, Genzor P, Feng X, Tsai P, Sun H, Haase AD, Sartorelli V, Hafner M |
| Citation(s) |
30146314 |
| |
| Submission date |
Jan 05, 2018 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Markus Hafner |
| E-mail(s) |
Markus.hafner@nih.gov
|
| Phone |
3014026956
|
| Organization name |
NIH
|
| Department |
NIAMS
|
| Lab |
Markus Hafner
|
| Street address |
50 South Drive
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21493 |
Illumina HiSeq 3000 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE108801 |
Nuclear miRNA-mediated gene silencing dependent on Argonaute proteins is required for embryonic stem cell differentiation |
|
| Relations |
| BioProject |
PRJNA428609 |
| SRA |
SRP128094 |
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