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| Status |
Public on Oct 01, 2018 |
| Title |
Human SETMAR is a DNA sequence-specific histone-methylase with a broad effect on the transcriptome |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Transposons impart dynamism to the genomes they inhabit and their movements frequently rewire the control of nearby genes. Occasionally, their proteins are domesticated when they evolve a new function. SETMAR is a protein methylase with a sequence-specific DNA binding domain. It began to evolve about 50 million years ago when an Hsmar1 transposon integrated downstream of a SET-domain methylase gene. Here we show that the DNA-binding domain of the transposase targets the enzyme to transposon-end remnants and that this is capable of regulating gene expression, dependent on the methylase activity. When SETMAR was modestly overexpressed in human cells, almost 1500 genes changed expression by more than 2-fold (65% up- and 35% down-regulated). These genes were enriched for the KEGG Pathways in Cancer and include several transcription factors important for development and differentiation. Expression of a similar level of a methylase-deficient SETMAR changed the expression of many fewer genes, 77% of which were down-regulated with no significant enrichment of KEGG Pathways. Our data is consistent with a model in which SETMAR is part of an anthropoid primate-specific regulatory network centered on the subset of genes containing a transposon end.
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| |
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| Overall design |
Examination of the effect of SETMAR or SETMAR N210A expression on U2OS transcriptome and ChIP-exo of Flag-SETMAR in U2OS cells.
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| Contributor(s) |
Tellier M, Chalmers R |
| Citation(s) |
30329085 |
| |
| Submission date |
Jan 04, 2018 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Michael Tellier |
| E-mail(s) |
mt477@leicester.ac.uk
|
| Organization name |
University of Leicester
|
| Department |
Department of Molecular and Cell Biology
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| Lab |
Tellier Lab
|
| Street address |
Lancaster Road
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| City |
Leicester |
| State/province |
Select State |
| ZIP/Postal code |
LE1 7HB |
| Country |
United Kingdom |
| |
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| Platforms (2) |
| GPL9442 |
AB SOLiD System 3.0 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA428518 |
| SRA |
SRP130934 |
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