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| Status |
Public on Feb 05, 2018 |
| Title |
Single-cell transcriptomics of the developing lateral geniculate nucleus reveals insights into circuit assembly and refinement |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Coordinated changes in gene expression underlie the early patterning and cell type specification of the central nervous system. However, much less is known about how such changes contribute to later stages of circuit assembly and refinement. In this study, we employ single-cell RNA-sequencing to develop a detailed, whole-transcriptome resource of gene expression across four time points in the developing dorsal lateral geniculate nucleus, a visual structure in the brain that undergoes a well-characterized program of postnatal circuit development. This approach identifies markers defining the major LGN cell types, including excitatory relay neurons, oligodendrocytes, astrocytes, microglia, and endothelial cells. Most cell types exhibit significant transcriptional changes across development, dynamically expressing genes involved in distinct processes including retinotopic mapping, synaptogenesis, myelination, and synaptic refinement. This transcriptomic resource provides a cellular map of gene expression across several cell types of the LGN, and offers insight into the molecular mechanisms of circuit development in the postnatal brain.
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| Overall design |
Examination of four developmental timepoints, each with four replicates. Raw data files were uploaded to SRA under SRP131204. The 6 multiplexed files were assigned SRA accession numbers SRR6510859, SRR6510864, SRR6510872, SRR6510876, SRR6510868, and SRR6510880.
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| Contributor(s) |
Kalish B, Greenberg M |
| Citation(s) |
29343640 |
| BioProject |
PRJNA431433 |
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| Submission date |
Jan 04, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Brian Kalish |
| E-mail(s) |
brian.kalish@sickkids.ca
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| Phone |
6305329007
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| Organization name |
Harvard Medical School
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| Department |
Neurobiology
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| Lab |
Greenberg Lab
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| Street address |
220 Longwood Ave
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (16)
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| Relations |
| SRA |
SRP131204 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE108761_LGN_SeqRuns.xlsx |
9.8 Kb |
(ftp)(http) |
XLSX |
| GSE108761_LGNdemultiplexing_1.txt.gz |
871 b |
(ftp)(http) |
TXT |
| GSE108761_RAW.tar |
191.0 Mb |
(http)(custom) |
TAR (of TSV) |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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