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Series GSE108746 Query DataSets for GSE108746
Status Public on Jan 18, 2019
Title High-dimensional transcriptional analyses of UC Colon comparing RNALater to DMSO collection method
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Simultaneous analyses of peripheral and mucosal immune compartments can yield insight into the pathogenesis of mucosal-associated diseases. Although methods to preserve peripheral immune cells are well established, studies involving mucosal immune cells have been hampered by lack of simple storage techniques. We provide a cryopreservation protocol allowing for storage of gastrointestinal (GI) tissue with preservation of viability and functionality of both immune and epithelial cells.
 
Overall design Multiple biopsies were taken from UC subjects to and placed into different collection media to compare the ability to extract total RNA and use total RNAseq to study the difference between inflamed and non-inflamed tissue
 
Contributor(s) Jelinsky S
Citation(s) 30111863
Submission date Jan 04, 2018
Last update date Mar 26, 2019
Contact name Scott Jelinsky
E-mail(s) Scott.Jelinsky@pfizer.com
Phone 617-674-7272
Organization name Pfizer
Department Inflammation and Immunology
Lab Computational Precision Medicine
Street address 610 Main Street
City Cambridge
State/province MA
ZIP/Postal code 02139
Country USA
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (72)
GSM2912835 inflamed rectum RNA Later 186931
GSM2912836 inflamed rectum DMSO: Intact Tissue 186931
GSM2912837 uninflamed ascending colon RNA Later 186931
Relations
BioProject PRJNA428476
SRA SRP128036

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE108746_data_matrix.txt.gz 4.6 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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