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| Status |
Public on Mar 18, 2008 |
| Title |
microRNA-155 is an Epstein-Barr Virus induced gene that modulates EBV-regulated gene expression (microRNA-155 infection) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The cellular microRNA, miR-155 has been shown to be involved in lymphocyte activation and is expressed in EBV infected cells displaying type III latency gene expression but not type I latency gene expression. We show here that the elevated levels of miR-155 in type III latency cells is due to EBV gene expression and not epigenetic differences in cell lines tested and we show that expression in EBV infected cells requires a conserved AP-1 element in the miR-155 promoter. Gene expression analysis was carried out in a type I latency cell line transduced with a miR-155 expressing retrovirus. This analysis identified both miR-155 suppressed and induced cellular mRNAs and suggested that in addition to direct targeting of 3’ UTRs, miR-155 alters gene expression in part through the alteration of signal transduction pathways. 3’ UTR reporter analysis of predicted miR-155 target genes identified the transcriptional regulatory genes, BACH1, ZIC3, HIVEP2, CEBPB, ZNF652, ARID2, and SMAD5 as miR-155 targets. Western blot analysis of the most highly suppressed of these, BACH1, showed lower expression in cells transduced with a miR-155 retrovirus. Inspection of the promoters from genes regulated in EBV infected cells and in cells infected with a miR-155 retrovirus identified potential binding sequences for BACH1 and ZIC3. Together, these experiments suggest that the induction of miR-155 by EBV contributes to EBV mediated signaling in part through the modulation of transcriptional regulatory factors.
Keywords: Differential expression of miR-155 vs cntl expressing cells
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| Overall design |
The EBV positive Burkitt's lymphoma cell line, Akata was infected with a control (pEhyg-miRCntl) or a microRNA-155 expressing (pEhyg-miR-155) retrovirus. Duplicate infections with the control retrovirus and with the miR-155 retrovirus were carried out. Control and miR-155 infection pair 1 were run on an array as well as a dye swap. Control and miR-155 infection pair 2 were similarly run on an array as well as a second array containing a dye swap.
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| Contributor(s) |
Yin Q, McBride J, Fewell C, Lacey M, Wang X, Lin Z, Cameron J, Flemington EK |
| Citation(s) |
18367535 |
| |
| Submission date |
Mar 17, 2008 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Erik K Flemington |
| E-mail(s) |
eflemin@tulane.edu
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| Phone |
504 988-1167
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| Organization name |
Tulane Health Sciences Center
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| Department |
Pathology
|
| Street address |
1430 Tulane Ave., SL79
|
| City |
New Orleans |
| State/province |
LA |
| ZIP/Postal code |
70112 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (4)
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| GSM275310 |
Akata miR-155 vs Akata cntl Infection pair 1 |
| GSM275359 |
Akata miR-155 vs Akata cntl Infection pair 2 |
| GSM275360 |
Akata cntl vs miR-155 Infection pair 1 (Dye Swap) |
| GSM275361 |
Akata cntl vs miR-155 Infection pair 2 (Dye Swap) |
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| This SubSeries is part of SuperSeries: |
| GSE10868 |
microRNA-155 is an Epstein-Barr Virus induced gene that modulates Epstein Barr virus regulated gene expression pathways |
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| Relations |
| BioProject |
PRJNA108887 |
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