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| Status |
Public on Jul 18, 2018 |
| Title |
RecBCD Coordinates Repair of Two Ends at a DNA Double-Strand Break, Preventing Aberrant Chromosome Amplification [MFA] |
| Organism |
Escherichia coli K-12 |
| Experiment type |
Other
|
| Summary |
The effect of an induced site-specific DNA double-strand break on DNA abundance across the chromosome of E. coli cells expressing or not RecD protein, was investigated by marker frequency analysis.
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| Overall design |
gDNA was isolated from two independent cultures ('biological repeats') of four, exponentially growing E. coli strains. Two strains experienced the induced DNA double-strand break (DL2006 and DL3391), two did not (DL2573 and DL3743). Two strains were wild-type for the recD gene (DL2006 and DL2573), two had the recD gene deleted (DL3391 and DL3743). gDNA was also isolated from two independent stationary phase cultures of strain DL2573. The libraries of the 10 gDNA preps were multiplexed and run 4 seperate times ('sequencing runs')
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| |
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| Contributor(s) |
White MA, Azeroglu B, Lopez-Vernaza MA, Mahedi Hasan AM, Leach D |
| Citation(s) |
29901759 |
| |
| Submission date |
Dec 12, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Benura Azeroglu |
| E-mail(s) |
b.azeroglu@ed.ac.uk
|
| Organization name |
University of Edinburgh
|
| Lab |
David Leach Lab
|
| Street address |
Alexander Crum Brown Road
|
| City |
Edinburgh |
| ZIP/Postal code |
EH9 3FF |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL19529 |
Illumina HiSeq 2000 (Escherichia coli K-12) |
|
| Samples (10)
|
| GSM2884558 |
DL2006 exponential growth biological repeat 1 |
| GSM2884559 |
DL2006 exponential growth biological repeat 2 |
| GSM2884560 |
DL2573 exponential growth biological repeat 1 |
| GSM2884561 |
DL2573 exponential growth biological repeat 2 |
| GSM2884562 |
DL3391 exponential growth biological repeat 1 |
| GSM2884563 |
DL3391 exponential growth biological repeat 2 |
| GSM2884564 |
DL3743 exponential growth biological repeat 1 |
| GSM2884565 |
DL3743 exponential growth biological repeat 2 |
| GSM2884566 |
DL2573 stationary phase biological repeat 1 |
| GSM2884567 |
DL2573 stationary phase biological repeat 2 |
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| This SubSeries is part of SuperSeries: |
| GSE107974 |
RecBCD Coordinates Repair of Two Ends at a DNA Double-Strand Break, Preventing Aberrant Chromosome Amplification |
|
| Relations |
| BioProject |
PRJNA422161 |
| SRA |
SRP126653 |
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