|
| Status |
Public on Apr 30, 2018 |
| Title |
Transcriptome analysis of human embryonic stem cells (hESCs) and human fibroblasts upon rapamycin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Rapamycin inhibits mTORC1 complex in a variety of cells. We demonstrated that hESCs are more sensitive to rapamycin treatment as compared to somatic cells (BJ Fibroblasts) and also certain cancer cells (KBM7 cells). To study the molecular mechanisms of this disparity, we performed a transcriptome analysis (RNAseq) of rapamycin-treated hESCs and human fibroblasts for two time points (2 and 4 days of treatment).
|
| |
|
| Overall design |
Human embryonic stem cells (hESCs) and human fibroblasts were treated with rapamycin for two and four days. For untreated controls and each treatment condition three biological replicates were analyzed.
|
| |
|
| Contributor(s) |
Yilmaz A, Peretz M, Benvenisty N |
| Citation(s) |
29662178 |
| |
| Submission date |
Dec 12, 2017 |
| Last update date |
Mar 26, 2019 |
| Contact name |
Mordecai Peretz |
| E-mail(s) |
motti.peretz@mail.huji.ac.il
|
| Organization name |
Hebrew University of Jerusalem
|
| Street address |
The Edmond J. Safra Campus
|
| City |
Jerusalem |
| ZIP/Postal code |
9190401 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA422061 |
| SRA |
SRP126557 |
Less...
More...