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| Status |
Public on Feb 16, 2018 |
| Title |
RNA-sequence analysis of immortalized, differentiated preadipocytes derived from inguinal subcutaneous white adipose tissue. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In acute cold stress in mammals, JMJD1A, an H3K9 demethylase, up-regulates thermogenic gene expressions through β-adrenergic signaling in brown adipose tissue (BAT). Aside BAT-driven thermogenesis, mammals also have another mechanism to cope with long-term cold stress by inducing the browning of subcutaneous white adipose tissue (scWAT). Here, we show that this occurs through a two-step process that requires both β-adrenergic dependent phosphorylation of S265 and demethylation of H3K9me2 by JMJD1A. The histone demethylation independent acute Ucp1 induction in BAT and demethylation dependent chronic Ucp1 expression in beige-scWAT provide complementary molecular mechanisms to ensure an ordered transition between acute and chronic adaptation to cold stress. JMJD1A mediates two major signaling pathways, namlely β-adrenergic receptor and PPARγ activation, via PRDM16-PPARγ-P-JMJD1A complex for beige adipogenesis.
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| Overall design |
Preadipocytes derived from inguinal subcutaneous white adipose tissue were immortalized. Mouse Jmjd1a was knocked-down by shRNA and wild-type and mutant versions of hJMJD1A were retrovirally expressed in preadipocytes. Preadipocytes were differentiated for RNA extraction and RNA-sequence analysis using Illumina HiSeq 2500.
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| Contributor(s) |
Sakai J |
| Citation(s) |
29674659, 38544573 |
| |
| Submission date |
Dec 11, 2017 |
| Last update date |
Apr 04, 2024 |
| Contact name |
Juro Sakai |
| E-mail(s) |
jmsakai-tky@umin.ac.jp
|
| Organization name |
Research Center for Advanced Science and Technology, The University of Tokyo
|
| Department |
Division of Metabolic Medicine
|
| Street address |
4-6-1, Komaba, Meguro-ku
|
| City |
Tokyo |
| ZIP/Postal code |
153-8904 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE107901 |
Histone demethylase JMJD1A coordinates acute and chronic adaptation to cold stress via thermogenic phospho-switch |
|
| Relations |
| BioProject |
PRJNA421901 |
| SRA |
SRP126488 |
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