|
| Status |
Public on Feb 20, 2018 |
| Title |
Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming [RNA-Seq-Cre] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
EBF1 is essential for B cell specification and commitment. To explore the dynamics of EBF1 initiated B cell programming, we performed EBF1 ChIP-seq, ATAC-seq, bisulfite-seq, RNA-seq and several histone ChIP-seq analyses at different stages of the transition from Ebf1-/- pre-pro-B to pro-B triggered by EBF1 restoration. We also performed Pax5 ChIP-seq in Ebf1-/- pre-pro-B cell and EBF1-restored pro-B cell to study the pioneering function of EBF1 that allows other transcription factors to access certain chromatin sites.
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| |
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| Overall design |
Time series RNA-Seq analysis during the differentiation from Ebf1-deficient pre-pro-B cell to EBF1-restored pro-B cell.
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| |
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| Contributor(s) |
Li R, Cauchy P, Ramamoorthy S, Lukas C, Grosschedl R |
| Citation(s) |
29440261 |
| |
| Submission date |
Nov 21, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Pierre Daniel Cauchy |
| E-mail(s) |
cauchy@ie-freiburg.mpg.de
|
| Phone |
+49 (0)761 270-77576
|
| Organization name |
University Medical Center Freiburg
|
| Department |
Zentrum für Translationale Zellforschung
|
| Lab |
AG Onco-Immunology
|
| Street address |
Breisacher Str. 115
|
| City |
Freiburg |
| State/province |
Baden-Württemberg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (8)
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| This SubSeries is part of SuperSeries: |
| GSE107242 |
Dynamic EBF1 occupancy directs sequential epigenetic and transcriptional events in B cell programming |
|
| Relations |
| BioProject |
PRJNA419402 |
| SRA |
SRP125420 |
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