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| Status |
Public on Jan 16, 2018 |
| Title |
Regional heterogeneity in response of airway epithelial cells to cigarette smoke |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To investigate the biochemical and genetic alterations that occur in response to cigarette smoke exposure among airway epithelial cells from different sites in the lungs, we performed microarray-based analysis using small airway epithelial cells (SAEC) and normal human bronchial epithelial cells (NHBE) following 24 h of cigarette smoke extract (CSE). In microarray-based analysis, the small airway showed higher susceptibility to CS compared to the large airway, such as enhanced expression of inflammatory-related pathways including the TNF signaling pathway. Among the TNF-related genes, PTGS2, also known as COX-2, showed the greatest difference in expression levels, with higher CSE-induced increments of both mRNA and protein expression in SAEC compared to NHBE.
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| Overall design |
SAEC and NHBE were subjected to 2.5% CSE for 24h. Microarray-based analysis was used to identify gene and protein expression profiles that are altered by CSE exposure in both cell types.
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| Contributor(s) |
Baskoro H, Sato T, Karasutani K, Suzuki Y, Mitsui A, Arano N, Nurwidya F, Kato M, Takahashi F, Kodama Y, Seyama K, Takahashi K |
| Citation(s) |
30180847 |
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| Submission date |
Nov 21, 2017 |
| Last update date |
May 20, 2019 |
| Contact name |
Tadashi Sato |
| E-mail(s) |
satotada@juntendo.ac.jp
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| Phone |
+81-3-5802-1063
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| Organization name |
Juntendo University Graduate School of Medicine
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| Department |
Department of Respiratory Medicine
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| Street address |
3-1-3 Hongo, Bunkyo-ku
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| City |
Tokyo |
| ZIP/Postal code |
113-8431 |
| Country |
Japan |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA419300 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE107200_RAW.tar |
24.5 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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