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| Status |
Public on May 10, 2018 |
| Title |
Transcriptional Analysis of Wild Type and KLF4-/- Peritoneal Macrophages |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Krüppel-like factor 4 (KLF4) is a critical regulator of macrophage activation. Here, we report transcriptomic differences in peritoneal macrophages isolated from Lysozyme M Cre (control) and myeloid-specific KLF4 knockout mice.
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| Overall design |
Peritoneal macrophages were isolated from lysozyme M Cre (wt) and myeloid-specific KLF4 knockout (K4KO) mice 72 hours after thioglycolate injection. Three mice per group were used. cDNA sequencing libraries were prepared using the Illumina TruSeq Stranded Total RNA kit and sequenced on a Illumina HiSeq 2500 platform. Reads from each sample were aligned to the mouse genome (UCSC mm10) using TopHat2 (Kim et al., 2013). Macrophage gene expression was quantitated as fragments per kilobase of transcript per million mapped reads (FPKM) using Cufflinks (Trapnell et al., 2010).
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| Contributor(s) |
Liao X, Tugal D, Fu C, Shen Y, Jain MK |
| Citation(s) |
29712858 |
| |
| Submission date |
Nov 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Mukesh Jain |
| Organization name |
Case Western Reserve University
|
| Street address |
2103 Cornell Rd
|
| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA418788 |
| SRA |
SRP125135 |
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