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Series GSE106991 Query DataSets for GSE106991
Status Public on Jan 09, 2018
Title RNA-seq analysis of human expandable arterial endothelial precursors (eAEPs), expandable mesenchymal precursors (eMPs), and controls
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary The following is an excerpt from the abstract of Miller et al. In this study, we find that two transcription factors, MYCN and SOX17, enable the indefinite propagation of human endothelial arterial precursors in vitro (“expandable arterial endothelial precursors,” eAEPs). Independent eAEP lines differ in their proclivity to undergo an endothelial-to-mesenchymal transition (EndoMT), a hallmark event in a broad array of vascular diseases and disorders. Some lines spontaneously become mesenchymal over time in culture, an effect exacerbated by inhibition of the fibroblast growth factor (FGF) receptor, while others do not readily convert. These distinctions were exploited to identify genes that correlate with resistance to an EndoMT and to elucidate transcriptional changes that underpin the transition. We believe the eAEPs may be useful not only as tractable tools for biological or pathological studies, but also as platforms for drug discovery.
Overall design Examination of eAEPs and eMPs, their mature progeny, and control vascular cells by RNA-seq.
Web link
Contributor(s) Vereide D
Citation(s) 29320761
NIH grant(s)
Grant ID Grant title Affiliation Name
U01 HL134655 Transplantation of MHC Homozygous Vascular Progenitors in Primates MORGRIDGE INSTITUTE FOR RESEARCH James Alexander Thomson
Submission date Nov 16, 2017
Last update date May 15, 2019
Contact name David Vereide
Organization name Morgridge Institute for Research
Department Regenerative Biology
Street address 330 North Orchard Street
City Madison
State/province WI
ZIP/Postal code 53715
Country USA
Platforms (2)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
GPL21290 Illumina HiSeq 3000 (Homo sapiens)
Samples (72)
GSM2858935 HAAF
GSM2858936 HBVP
GSM2858937 HUVEC_1
BioProject PRJNA418736
SRA SRP125103

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Supplementary file Size Download File type/resource
GSE106991_ExpectedCounts.txt.gz 1.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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