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| Status |
Public on Jan 09, 2018 |
| Title |
RNA-seq analysis of human expandable arterial endothelial precursors (eAEPs), expandable mesenchymal precursors (eMPs), and controls |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The following is an excerpt from the abstract of Miller et al. In this study, we find that two transcription factors, MYCN and SOX17, enable the indefinite propagation of human endothelial arterial precursors in vitro (“expandable arterial endothelial precursors,” eAEPs). Independent eAEP lines differ in their proclivity to undergo an endothelial-to-mesenchymal transition (EndoMT), a hallmark event in a broad array of vascular diseases and disorders. Some lines spontaneously become mesenchymal over time in culture, an effect exacerbated by inhibition of the fibroblast growth factor (FGF) receptor, while others do not readily convert. These distinctions were exploited to identify genes that correlate with resistance to an EndoMT and to elucidate transcriptional changes that underpin the transition. We believe the eAEPs may be useful not only as tractable tools for biological or pathological studies, but also as platforms for drug discovery.
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| |
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| Overall design |
Examination of eAEPs and eMPs, their mature progeny, and control vascular cells by RNA-seq.
|
| Web link |
http://dx.doi.org/10.1016/j.stemcr.2017.12.011
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| |
|
| Contributor(s) |
Vereide D |
| Citation(s) |
29320761 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| U01 HL134655 |
Transplantation of MHC Homozygous Vascular Progenitors in Primates |
MORGRIDGE INSTITUTE FOR RESEARCH |
James Alexander Thomson |
|
| Submission date |
Nov 16, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
David Vereide |
| E-mail(s) |
dvereide@morgridge.org
|
| Organization name |
Morgridge Institute for Research
|
| Department |
Regenerative Biology
|
| Street address |
330 North Orchard Street
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53715 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
|
| Samples (72)
|
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| Relations |
| BioProject |
PRJNA418736 |
| SRA |
SRP125103 |
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