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| Status |
Public on Nov 15, 2017 |
| Title |
Epigenome-wide Effects of Vitamin-D on Bronchial Epithelial Cells using ATAC-Seq and RNA-seq |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Vitamin D has been associated with viral respiratory infections, the main cause of severe asthma exacerbations in children. We used ATAC-Seq to evaluate the effect of vitamin D on chromatin accessibility in immortalized (BEAS-2B), normal (NHBEC), and asthma (AAEC) bronchial epithelial cells cultured and stimulated with calcitriol, poly I:C (to simulate viral infection), both, or sham (culture media), as well as the effect on gene expression.
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| Overall design |
ATAC-Seq and total RNA-Seq were performed on 4 asthma human bronchial epithelial cell samples (AAEC), 4 normal human bronchial epithelial cell samples (NHBEC) and 4 immortalized (BEAS-2B) samples, respectively treated with calcitriol, poly I:C, both or sham (culture media).
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| |
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| Contributor(s) |
Celedon JC, Wang T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 14, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Ting Wang |
| E-mail(s) |
wang9ting@gmail.com
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| Organization name |
Fred Hutchinson Cancer Research Center
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| Department |
Clinical Research
|
| Street address |
1100 Fairview Ave. N.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA418319 |
| SRA |
SRP124965 |
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