|
| Status |
Public on Jan 01, 2019 |
| Title |
mTORC2/Akt Activation in Adipocytes is Required for Mycobacterium tuberculosis Derived Inflammation in Adipose Tissue |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The association between type 2 DM (T2DM) and susceptibility to tuberculosis (TB) is well recognized. Patients with DM have 2-3 fold increased susceptibility to TB, treatment is longer and the outcome is worse than in non-diabetic TB patients. Here, we report that aerosol infection with M. tuberculosis is followed by leukocyte infiltration and proliferation, and expression of inflammatory markers in adipose tissue of lean mice leading to disrupted lipid metabolism and the induction of adipocyte hypertrophy. RNA-sequencing (RNAseq) in whole pgWAT adipose tissue at 8 wk after aerosol challenge revealed an upregulation of genes that were associated with antigen presentation, Th1 and type-1 interferon response, indicated the induction of T cell activation. This was found to be due to activated mTORC2/Akt signaling. By depleting Rictor (mTORC2 subunit) in adipocytes we noticed the restoration of inflammation and cell hypertrophy. Our study suggests that TB-induced inflammation in the adipose tissue is associated with improved glucose tolerance and is mediated by mTORC2/Akt pathway.
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| Overall design |
primary murine adipose tissue from 5 uninfected and 6 Tb infected mice 8 weeks post infection
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| Contributor(s) |
Singhal A, Poidinger M |
| Citation missing |
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| |
| Submission date |
Nov 07, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael Poidinger |
| E-mail(s) |
michael_poidinger@immunol.a-star.edu.sg
|
| Phone |
+6564070528
|
| URL |
http://www.sign.a-star.edu.sg
|
| Organization name |
Singapore Immunology Network
|
| Department |
Bioinformatics
|
| Street address |
#04-06, 8A Biomedical Grove
|
| City |
Singapore |
| ZIP/Postal code |
138648 |
| Country |
Singapore |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA417450 |
| SRA |
SRP124469 |
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