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| Status |
Public on Apr 20, 2020 |
| Title |
Systemic inflammation drives rapid conversion of conventional CD4+ T cells to Treg in vivo |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The immune system faces a task that approximates cognition in its complexity as it processes a multitude of intrinsic and extrinsic signals and integrates these into responses of the appropriate class, specificity, magnitude, and duration for a given threat, with the host’s life often depending on the outcome. CD4+ T lymphocytes occupy a unique role in the generation and regulation of immunity within this context and influence multiple innate and adaptive cell types. With respect to CD8+ cytotoxic T lymphocytes (CTL), CD4+ T cells function early in the response as ‘helpers’ (TH) to increase its magnitude and functionality, and later as regulatory cells (Treg) to restore homeostasis and avoid immune pathology. Using a Listeria monocytogenes (Lm) infection model, we probed whether the conditions of initial pathogen encounter could influence the generation of TH versus Treg. At low dose infection, CD4+ T cells ‘help’ CTLs via CD40-CD40L signaling, while high dose infection induces rapid polyclonal conversion to functional FoxP3+CD25+ Treg within 24 hours. These findings resolve long-standing questions regarding the requirement for TH and reveal a remarkable degree of plasticity in the function of CD4+ T cells, which can be quickly converted to Treg in vivo in response to acute inflammation.
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| Overall design |
Differential gene expression analysis of total RNA isolated from splenic CD4+eGFP− (Tconv) and CD4+eGFP+ (Treg) from Foxp3eGFP mice following low dose and high dose Lm ΔactA-Ova infections.
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| Contributor(s) |
Dolina JS, Lee J, Labarta-Bajo L, Kannan S, Greenbaum JA, Mikulski Z, Bahia El Idrissi N, Pont MJ, Rosales SL, Seumois G, Vijayanand P, Croft M, Schoenberger SP |
| Citation missing |
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| Submission date |
Nov 06, 2017 |
| Last update date |
Apr 21, 2020 |
| Contact name |
Joseph Samuel Dolina |
| Organization name |
La Jolla Institute for Allergy and Immunology
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| Street address |
9420 Athena circle
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| City |
San Diego |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA417240 |
| SRA |
SRP124228 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106554_All_HTSeq_Counts.tsv.gz |
298.4 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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