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| Status |
Public on Sep 25, 2018 |
| Title |
Neonatal imprinting of mesenteric lymph node stroma modulates tolerogenic properties of dendritic cells [scRNA-seq] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Gut-draining mesenteric lymph nodes (mLNs) play a key role in peripheral tolerance towards food and commensal antigens by providing an optimal microenvironment for efficient de novo induction of Foxp3+ regulatory T cells (Tregs). We recently identified mLN stromal cells as critical cellular players in this process and demonstrated that their tolerogenic properties are imprinted by microbiota. Here, we show that this imprinting process already takes place in the neonatal phase and renders the mLN stromal cell compartment resistant to inflammatory perturbations later in life. Utilizing LN transplantation, RNA-seq and single-cell RNA-seq allowed identification of stably imprinted expression signatures in mLN fibroblastic stromal cells. We dissected common stromal cell subsets across gut-draining mLNs and skin-draining LNs with location-specific immunomodulatory functions, such as subset-specific expression of Aldh1a2/3. Accordingly, mLN stromal cells shaped resident dendritic cells to attain high Treg-inducing capacity in a Bmp2-dependent manner. Thus, crosstalk between mLN stromal and resident dendritic cells provides a robust feedback mechanism for the maintenance of intestinal tolerance.
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| Overall design |
Single-cell transcriptomic analysis of skin-draining pLNs (inguinal and axillary) or mLNs (small intestinal and colon/caecum-draining) cell populations.
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| Contributor(s) |
Pezoldt J, Pasztoi M, Zou M, Wiechers C, Beckstette M, Thierry G, Vafadarnejad E, Floess S, Arampatzi P, Buettner M, Schweer J, Fleissner D, Vital M, Pieper DH, Basic M, Dersch P, Strowig T, Hornef M, Bleich A, Bode U, Pabst O, Bajenoff M, Saliba A, Huehn J |
| Citation(s) |
36227687 |
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| Submission date |
Nov 03, 2017 |
| Last update date |
Jan 05, 2023 |
| Contact name |
Joern Pezoldt |
| E-mail(s) |
jorn.pezoldt@epfl.ch
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| Phone |
0041766040171
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| Organization name |
EPFL
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| Department |
SV
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| Lab |
Laboratory Systems Biology and Genetics
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| Street address |
Station 19, SV 3818.A
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| City |
Lausanne |
| ZIP/Postal code |
1015 |
| Country |
Switzerland |
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| Platforms (2) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE116633 |
Neonatally imprinted mesenteric lymph node stromal cell subsets induce tolerogenic dendritic cells |
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| Relations |
| BioProject |
PRJNA417026 |
| SRA |
SRP123573 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106489_DE-MULTIPLEXING_README.txt |
162 b |
(ftp)(http) |
TXT |
| GSE106489_DE-MULTIPLEXING_README_Bsamples.txt |
150 b |
(ftp)(http) |
TXT |
| GSE106489_RAW.tar |
996.3 Mb |
(http)(custom) |
TAR (of CSV, H5) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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