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| Status |
Public on Nov 22, 2017 |
| Title |
Gene expression analysis in fly ovaries carrying Sco homozgyous clones |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: The goal of this study is to analyze genes affected by Sco mutation using RNA-seq.
Ovarian mRNA prolife of 7-day-old control flies and flies carring Sco mutant follicle cell clones were generated by deep sequence, in duplicate. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks.
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| Overall design |
Ovarian mRNA prolife of 7-day-old control flies and flies carring Sco mutant follicle cell clones were generated by deep sequence, in duplicate. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods:
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| Contributor(s) |
Hsu H, Tseng C |
| Citation(s) |
29261681 |
| |
| Submission date |
Nov 03, 2017 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Hweijan Hsu |
| E-mail(s) |
cohsu@gate.sinica.edu.tw
|
| Phone |
886-2-2787-1541
|
| Organization name |
Academia Research
|
| Department |
Institute of Cellular Biology
|
| Lab |
Room 337
|
| Street address |
no 128, Sec 2, Academia Rd. Nankang
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA417023 |
| SRA |
SRP123572 |
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