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| Status |
Public on Jul 16, 2018 |
| Title |
Gene expression analyses of mouse mesenchymal cells, keratinocytes and induced stratified epithelial progenitor cells. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the method for generating epithelial tissues from bottom of ulcer by lineage conversion. Both in vitro and in vitro, mesenchymal cells can be converted to the cells having ability to form stratified epithelium by transduction of lineage-specific transcription factors. We named the converted cells as induced stratified epithelial progenitor cells (iSEPs). Gene expression signatures of mesenchymal cells, in vitro generated iSEPs, in vivo generated iSEPs, and primary keratinocytes are analyzed.
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| Overall design |
Three sets of Pdgfra (+) adipose derived stromal cells (ADSCs), in vitro iSEPs, in vivo iSEPs, and primary keratinocytes are prepared from 3 nimals.
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| Contributor(s) |
Belmonte JI, Shokhirev MN, Kurita M |
| Citation(s) |
30185909 |
| |
| Submission date |
Nov 01, 2017 |
| Last update date |
Mar 28, 2022 |
| Contact name |
April Elizabeth Williams |
| E-mail(s) |
apriljack06@gmail.com, awilliams@salk.edu
|
| Phone |
7345461645
|
| Organization name |
Salk Institute for Biological Studies
|
| Department |
IGC
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
San Diego |
| State/province |
California |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA416808 |
| SRA |
SRP123356 |
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