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Series GSE106400 Query DataSets for GSE106400
Status Public on Mar 23, 2018
Title Gene expression profile of Ba/F3 variant (#BEB1) cells that have binding ability to Ecrg4
Organism Mus musculus
Experiment type Expression profiling by array
Summary Esophageal cancer-related gene 4 (Ecrg4) encodes a hormone-like peptide, and is thought to be involved in a variety of physiological phenomena including tumor suppressor function. Recent progress of Ecrg4 reveals that Ecrg4 peptides are pro-inflammatory and induce the expression of several cytokines and chemokines. However, detailed molecular mechanisms of Ecrg4 signaling, especially the receptors for Ecrg4 peptides, remain poorly understood. Here, using retrovirus-mediated expression cloning, we identified lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) as a membrane protein bound for amino acid residues 71-132 of Ecrg4, Ecrg4(71-132). Moreover, not only LOX-1 but also several scavenger receptors such as Scarf1, Cd36 and Stabilin-1 efficiently internalized Ecrg4(71-132) into cells. The broad scavenger receptor competitive inhibitor polyinosinic acid reduced both the internalization of Ecrg4(71-132) and activation of NF-kB in microglia. In addition, this activation is dependent on Myd88, an adaptor protein to recruit signaling proteins to TLR and IL-1R receptors with induction of several immune response. These data suggest that multiple scavenger receptors recognize Ecrg4(71-132) and transduce its signals along with TLR/IL-1R receptors in microglia.
Overall design A variant strain of Ba/F3 (#BEB1) cells that have an ability to bind Ecrg4 was obtained after five round sorting using retrovirus expression library prepared from mRNA of CG4 cells.
Contributor(s) Enomoto K, Moriguchi T
Citation(s) 29511297
Submission date Nov 01, 2017
Last update date Mar 24, 2018
Contact name Kei Enomoto
Organization name ASUBIO PHARMA CO., LTD.
Street address Chuo-ku, Minatojima-Minamimachi
City Kobe
State/province Hyogo
ZIP/Postal code 650-0047
Country Japan
Platforms (1)
GPL11202 Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version)
Samples (2)
GSM2837207 Ba/F3_control
GSM2837208 Ba/F3_#BEB1
BioProject PRJNA416768

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106400_RAW.tar 18.0 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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