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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 23, 2018 |
| Title |
Gene expression profile of Ba/F3 variant (#BEB1) cells that have binding ability to Ecrg4 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Esophageal cancer-related gene 4 (Ecrg4) encodes a hormone-like peptide, and is thought to be involved in a variety of physiological phenomena including tumor suppressor function. Recent progress of Ecrg4 reveals that Ecrg4 peptides are pro-inflammatory and induce the expression of several cytokines and chemokines. However, detailed molecular mechanisms of Ecrg4 signaling, especially the receptors for Ecrg4 peptides, remain poorly understood. Here, using retrovirus-mediated expression cloning, we identified lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) as a membrane protein bound for amino acid residues 71-132 of Ecrg4, Ecrg4(71-132). Moreover, not only LOX-1 but also several scavenger receptors such as Scarf1, Cd36 and Stabilin-1 efficiently internalized Ecrg4(71-132) into cells. The broad scavenger receptor competitive inhibitor polyinosinic acid reduced both the internalization of Ecrg4(71-132) and activation of NF-kB in microglia. In addition, this activation is dependent on Myd88, an adaptor protein to recruit signaling proteins to TLR and IL-1R receptors with induction of several immune response. These data suggest that multiple scavenger receptors recognize Ecrg4(71-132) and transduce its signals along with TLR/IL-1R receptors in microglia.
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| Overall design |
A variant strain of Ba/F3 (#BEB1) cells that have an ability to bind Ecrg4 was obtained after five round sorting using retrovirus expression library prepared from mRNA of CG4 cells.
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| Contributor(s) |
Enomoto K, Moriguchi T |
| Citation(s) |
29511297 |
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| Submission date |
Nov 01, 2017 |
| Last update date |
Mar 24, 2018 |
| Contact name |
Kei Enomoto |
| E-mail(s) |
enomoto.kei.yn@asubio.co.jp
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| Organization name |
ASUBIO PHARMA CO., LTD.
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| Street address |
Chuo-ku, Minatojima-Minamimachi
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| City |
Kobe |
| State/province |
Hyogo |
| ZIP/Postal code |
650-0047 |
| Country |
Japan |
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| Platforms (1) |
| GPL11202 |
Agilent-026655 Whole Mouse Genome Microarray 4x44K v2 (Probe Name version) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA416768 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE106400_RAW.tar |
18.0 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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