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| Status |
Public on Jan 31, 2018 |
| Title |
The histone acetyl-tranferase MOF is required for metabolic function in the liver . |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: Conditional MOF deletion in mouse livers resulted in acute hepatic necrosis. The goal of this study was to identify transcriptomic changes in the livers of MOF-depeleted mice livers compared to WT.
Methods: Transcriptomic profiles of MOF∆ and WT mouse livers were generated in triplicate. Sequenced reads were quality filtered using Trimmomatic. Quality reads were aligned to the mouse genome using TopHat2. Reads were counted using htseq-count and differentially expressed transcripts were identified using DESeq.
Results: 1408 differentially expressed genes were identified between MOF∆ and WT livers. 774 genes were down-regulated in MOF∆ and primarilly associated with metabolic pathways. Branched chain amino acid and fatty acid metabolic pathways, which are deregulated in non-alcoholic fattly liver disease (NAFLD) prgression were down-regulated. 664 genes were upregulated and associated with increased proliferation.
Conclusions: These data show that MOF is required for maintaining proper metabolic function in the liver and deregulation of MOF may be involved in progression of NAFLD.
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| Overall design |
Liver mRNA profiles of 6-8 week old old wild type (WT) and MOF∆ mice were generated by deep sequencing, in triplicate, using the Illumina HiSeq 4000 platform.
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| Contributor(s) |
denDekker AD, Dou Y |
| Citation(s) |
33376138 |
| |
| Submission date |
Oct 31, 2017 |
| Last update date |
Mar 23, 2021 |
| Contact name |
Aaron David denDekker |
| E-mail(s) |
adendekk@med.umich.edu
|
| Organization name |
University of Michigan
|
| Department |
Pathology
|
| Lab |
5215 Med Sci I
|
| Street address |
1301 Catherine Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA416459 |
| SRA |
SRP123068 |
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