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Series GSE106361 Query DataSets for GSE106361
Status Public on Feb 04, 2020
Title Differential Notch target genes expression profile upon HDAC3 loss-of-function.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The Notch signaling pathway regulates several differentiation and developmental processes in both pre- and post-natal life and its aberrant regulation leads to diseases, including cancer. Here, we investigated the role of histone deacetylase 3 (HDAC3) on the regulation of the Notch-dependent gene expression program in mouse pre-T (Beko) cells. We first defined Notch target genes as those genes that are downregulated upon inhibition of the Notch pathway using DAPT, a gamma-secretase inhibitor (GSI). Subsequently, we investigated the role of HDAC3 in the regulation of Notch target genes by shRNA- and pharmacologically (apicidin)-mediated loss-of-function of HDAC3.
Overall design Mouse pre-T cells (Beko) were treated with gamma-secretase inhibitor (GSI) DAPT to identify Notch target genes by RNA-seq analysis. The function of HDAC3 at Notch target genes was investigated by shRNA- or pharmacological (Apicidin)-mediated loss-of-function of HDAC3 followed by RNA-seq analysis.
Contributor(s) Bartkuhn M, Ferrante F, Giaimo BD, Borggrefe T
Citation(s) 32107550
Submission date Oct 30, 2017
Last update date Apr 20, 2020
Contact name Marek Bartkuhn
Organization name Justus-Liebig-University Giessen
Department Biomedical Informatics and Systems Medicine
Street address Aulweg 132
City Giessen
State/province Hessen
ZIP/Postal code 35392
Country Germany
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (12)
GSM2836561 Beko_Apicidin_rep1
GSM2836562 Beko_Apicidin_rep2
GSM2836563 Beko_DMSO_(Apicidin_Ctrl)_rep1
BioProject PRJNA416379
SRA SRP122989

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Supplementary file Size Download File type/resource
GSE106361_Apicidin_vs_control.txt.gz 1.2 Mb (ftp)(http) TXT
GSE106361_DAPT_vs_control.txt.gz 1.3 Mb (ftp)(http) TXT
GSE106361_shHdac3_vs_control.txt.gz 1.2 Mb (ftp)(http) TXT
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Processed data are available on Series record

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