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| Status |
Public on Sep 28, 2018 |
| Title |
Transcriptome of lung tissue from C57BL/6 mice with or without neutrophil depletion at ZT04 and ZT16 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Our study looks at the dirsruption of lung circadian transcriptome that occurs when neutrophils are depleted (by application of antibodies (anti-Ly6G-1A8) to wildtype C57BL/6 mice, or Diphtheria toxin (DT) to neutrophil-specific DT-susceptible mice (MRP8-Cre;iDTR-flox)).
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| Overall design |
Lungs were harvested from neutrophil-depleted (antibody or DT) or non-depleted mice, in normal light-controlled mouse facility (ZT4) or 12h inverted light cabinets (ZT16). Experiments were carried out over 3 batches (July 2017, September 2017, and April 2018), with 3 or 4 mice per group. Antibody-depleted and non-depleted mice were tested for the July 2017 and September 2017 batches, whereas DT-depleted mice were tested only in April 2018.
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| Contributor(s) |
Hidalgo A, Li JL |
| Citation(s) |
30282719 |
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| Submission date |
Oct 30, 2017 |
| Last update date |
May 09, 2024 |
| Contact name |
Andrés Hidalgo |
| E-mail(s) |
andres.hidalgo@yale.edu
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| Organization name |
Yale University
|
| Street address |
10 Amistad Street
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| City |
New Haven |
| ZIP/Postal code |
06519 |
| Country |
USA |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (35)
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| Relations |
| BioProject |
PRJNA416366 |
| SRA |
SRP122966 |
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