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| Status |
Public on Jun 01, 2018 |
| Title |
Multi-axial self-organisation properties of mouse embryonic stem cells into gastruloids [individual] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Gastruloids, small aggregates of murine Embryonic Stem (ES) cells stimulated to undergo gastrulation-like symmetry breaking events in vitro (van den Brink, S. C. et al. 2014, Simunovic, M. & Brivanlou, A. H 2017). Analysis of the transcriptional profiles from pools of gastruloids collected at different time-points after aggregation (AA) revealed that gastruloids are capable of organizing an axially integrated post-occipital pattern of neural, mesodermal and endodermal derivatives that mimics that of the embryo in terms of gene expression. To test the homogeneity and reproducibility of the transcriptional programs uncovered by our bulked RNAseq experiments, we performed RNAseq analysis of individual gastruloids collected at different time-points AA (10 biological replicates per time-point). Our results indicates that the transcriptional profiles of gastruloid were highly reproducible at each time points and that each gastruloid implement the full diversity of transcriptional programs detected in our bulked RNAseq experiments.
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| Overall design |
RNA-seq analysis of individual gastruloids collected at different time-points after aggregation
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| Contributor(s) |
Beccari L, Moris N, Girgin M |
| Citation missing |
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| |
| Submission date |
Oct 26, 2017 |
| Last update date |
Nov 11, 2023 |
| Contact name |
Leonardo Beccari |
| E-mail(s) |
leonardo.beccari@unige.ch
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| Organization name |
University of Geneva
|
| Department |
Genetics and Evolution
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| Street address |
30 Quai Ernest Ansermet
|
| City |
Geneva |
| ZIP/Postal code |
1211 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (30)
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| This SubSeries is part of SuperSeries: |
| GSE106227 |
Multi-axial self-organisation properties of mouse embryonic stem cells into gastruloids |
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| Relations |
| BioProject |
PRJNA415971 |
| SRA |
SRP121671 |
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