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| Status |
Public on Jul 08, 2018 |
| Title |
H3K9me3 and H3K36me3 ChIP-seq in WT and H3.3 G34R mouse ES cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Sequencing of paediatric gliomas has identified two common substitution mutations (K27M and G34R) in genes encoding histone H3.3. We introduced a single-copy H3.3 G34R targeted mutation in mouse ES cells and observed gains in H3K36me3 and H3K9me3 across the genome. Altered chromatin profiles correlated with enrichment of KDM4 A/B/C, a histone lysine (K9/K36) demethylase. RNA-seq of H3.3 G34R mutant showed disrupted gene expression patterns which also correlated with KDM4 enrichment. Expression of a single copy of H3.3 G34R at endogenous levels was sufficient to genocopy KDM4 triple-KO cells as determined by ChIP-seq and RNA-seq.
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| |
|
| Overall design |
4 samples - ChIP-seq of H3K9me3 and H3K36me3 in WT control and H3.3 G34R mutant cells
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| |
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| Contributor(s) |
Voon HP, Wong LH |
| Citation(s) |
30087349 |
| |
| Submission date |
Oct 26, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Hsiao Phin Joanna Voon |
| E-mail(s) |
Joanna.Voon@monash.edu
|
| Organization name |
Monash University
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Epigenetics and Chromatin
|
| Street address |
Monash University, Wellington Road
|
| City |
Clayton |
| State/province |
Victoria |
| ZIP/Postal code |
3800 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE106205 |
The H3.3 G34R glioma-associated mutation alters H3K9me3 and H3K36me3 chromatin modifications |
|
| Relations |
| BioProject |
PRJNA415918 |
| SRA |
SRP121629 |
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