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Series GSE106203 Query DataSets for GSE106203
Status Public on Jul 08, 2018
Title H3K9me3 and H3K36me3 ChIP-seq in WT and H3.3 G34R mouse ES cells
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Sequencing of paediatric gliomas has identified two common substitution mutations (K27M and G34R) in genes encoding histone H3.3. We introduced a single-copy H3.3 G34R targeted mutation in mouse ES cells and observed gains in H3K36me3 and H3K9me3 across the genome. Altered chromatin profiles correlated with enrichment of KDM4 A/B/C, a histone lysine (K9/K36) demethylase. RNA-seq of H3.3 G34R mutant showed disrupted gene expression patterns which also correlated with KDM4 enrichment. Expression of a single copy of H3.3 G34R at endogenous levels was sufficient to genocopy KDM4 triple-KO cells as determined by ChIP-seq and RNA-seq.
Overall design 4 samples - ChIP-seq of H3K9me3 and H3K36me3 in WT control and H3.3 G34R mutant cells
Contributor(s) Voon HP, Wong LH
Citation(s) 30087349
Submission date Oct 26, 2017
Last update date May 15, 2019
Contact name Hsiao Phin Joanna Voon
Organization name Monash University
Department Biochemistry and Molecular Biology
Lab Epigenetics and Chromatin
Street address Monash University, Wellington Road
City Clayton
State/province Victoria
ZIP/Postal code 3800
Country Australia
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (4)
GSM2831539 WT129_H3K9me3_ChIPSeq
GSM2831540 G34R_H3K9me3_ChIPSeq
GSM2831541 WT129_H3K36me3_ChIPSeq
This SubSeries is part of SuperSeries:
GSE106205 The H3.3 G34R glioma-associated mutation alters H3K9me3 and H3K36me3 chromatin modifications
BioProject PRJNA415918
SRA SRP121629

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE106203_H3K36me3_enriched_regions.txt.gz 188.6 Kb (ftp)(http) TXT
GSE106203_H3K9me3_enriched_regions.txt.gz 202.5 Kb (ftp)(http) TXT
GSE106203_RAW.tar 318.1 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

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